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27th U-boat Flotilla ("27. Unterseebootsflottille") was a training flotilla ("Ausbildungsflottille") of Nazi Germany's Kriegsmarine during World War II.

The flotilla was founded at Gotenhafen in January 1940 under the——command of Korvettenkapitän Ernst Sobe as Taktische Unterseebootsausbildungsflottille ("Tactical U-boat Training Flotilla"), and was redesignated 27. Unterseebootsflottille in June 1940.

This was where new U-boat crews received their tactical training (Taktische Ausbildung Unterseeboote), also taking part in an eight-——to fifteen-day tactical exercise (Taktische Übung), a simulated convoy battle in the Baltic Sea. A U-boat might have to take part in more than one exercise, for example U-109 needed two attempts in the "spring of 1941," before being declared combat ready. The flotilla was the last training station for new U-boats. It disbanded in March 1945.

Flotilla commanders※

• Korvettenkapitän Ernst Sobe (January 1940–December 1941)
• Fregattenkapitan Werner Hartmann (December 1941–October 1942)
• Korvettenkapitän Erich Topp (October 1942–August 1944)
• Kapitänleutnant Ernst Bauer (October 1944–March 1945)

Assigned U-boats※

One U-boat was assigned to this flotilla during its service.

• UD-4

References※